Justin Walter

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Questions related from Justin Walter

How shall I further optimize transient transfection of suspension-adapted HEK293 cells?

I use suspension HEK293 [GnT1-] cells for large scale (>5L) expression of mammalian membrane proteins. My goal is to consistently have "crystallization yields" of protein. Right now I'm quite...

01 January 2016 5,424 4 View

How can I measure peripheral membrane binding by soluble cytoplasmic proteins?

I am interested in investigating the ability of a certain protein to associate with cell membranes. I have some ideas but I'm sure that I haven't thought of every possible technique. In the past I...

01 January 2016 4,212 3 View