Justin Andrew Smith

3 Questions 12 Answers 0 Followers

Questions related from Justin Andrew Smith

When analyzing images of fibers in coronally-sectioned mouse brain, is it possible to differentiate between fibers of passage and terminal fibers?

Does anyone have a method to determine the difference by morphology alone i.e. without the use of an additional label such as synapsin for terminals or tau for axons? Our neurons are filled with...

04 April 2015 1,172 3 View

Tracer injection analysis in mouse brain tissue.

Hello, We are injecting tracer into the mouse brain using a picospritzer. Our target region is the bed nucleus of the stria terminalis which is in close proximity to the lateral ventricles. My...

08 August 2014 1,126 18 View

Does anyone have a procedure for determining optimal fixation of mouse brain sections for IHC?

I saw a poster at a conference that presented data suggesting that by altering the pH and/or the volume of 4% PFA during transcardial perfusion it is possible to tailor the fix for optimal...

07 July 2014 660 9 View