Justin Lawrie

2 Questions 2 Answers 0 Followers

Questions related from Justin Lawrie

Why do I only get template plasmid after performing Kunkel mutagenesis and electroporation?

I am performing Kunkel mutagenesis using three (non-overlapping) primers to introduce soft randomizations into my protein of interest. Based on an agarose gel it appears the reaction has occurred...

06 February 2020 4,269 0 View

Why isn't my phage displaying my phage library following electroporation?

I have been trying to screening a library of mutant proteins for affinity for a similar ligand as its natural binding ligand. However, currently I have been unsuccessful in this endeavor even...

24 July 2019 6,909 5 View