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Can YNB be avoided in rich medium such as BMMY for protein expression (I am working with P.Pastoris), since there are already nitrogen sources and vitamins from yeast extract and peptone?
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I am designing protein expression in Pichia Pastoris GS115 Mut+. I wonder which is the best way to add MeOH; either once every 24h to final 1% concentration (pulsed mode) or continually at some...
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