Why there are only 3 (not 4) clusters present on my ddPCR EvaGreen different primer concentration duplex assay results?

Hi, I have recently ran a duplex ddPCR EvaGreen assay using different primer concentration for target (150nM) and reference (50nM) primer. The assay was ran on a XY sample for SRY copy number...

23 June 2024 9,071 3 View