Junyeol Han

4 Questions 7 Answers 0 Followers

Questions related from Junyeol Han

Do you know why Delta Ct values variation occur in the control sample with each attempt ?

Hello, I have a question. I'm performing qPCR experiments, and while repeating the experiment several times, I notice that the Delta Ct values for the housekeeping gene and target gene in the...

12 November 2024 8,197 6 View

Is there any experience to isolate mouse lung endothelial cells using MACS?

Hello, is there any experience to isolate mouse lung endothelial cells using MACS? I have troubles about viability and purity about the cells. I have tried to isolate and culture mouse lung...

23 August 2024 2,719 4 View

When you guys want to deplete bmp4, you usually do not use bmp4 gene knockdown directly?

I recently start and try to research the Bone morphogenetic protein 4 (bmp4) gene. I want to inhibit BMP4 gene expression and so I think bmp4 gene knockdown using si or shRNA is okay. but when I...

09 March 2024 5,236 0 View

What is the appropriate G418 concentration and time for selection about Mlg cell line?

Hi, I' am trying to make stable cell line using plasmid transfection. the plasmid are available for neomycin selection. I want to know the appropriate G418 concentration and time forselection...

01 March 2024 8,428 2 View