Junsoung Kwak

3 Questions 11 Answers 0 Followers

Questions related from Junsoung Kwak

What is the problem at non-radioactive method for miRNA detection by northern blot?

Hello I did several times to detect the miRNA by northern blot. Briefly explain my protocol. Small RNA was prepared by extraction kit and load the 2.5ug of RNA on 15% TBE-urea gel 1hour. After...

10 October 2018 9,220 9 View

How do I perform a CRISPR CAS9 knock out confirmation ?

Hello i am doing an experiment knock out a gene in epithelioma papulosum cyprini (EPC) cell using precision cas9 smartnuclease vector system from system bioscience. following the guidelines, i...

02 February 2015 3,310 4 View

Is the T7E1 assay very sensitive?

Hello I used crispr cas9 system to knock out gene. I transfected vector (SBI) to EPC cell line and selected cell and extract gDNA from 1x10^6 cells/ml and did PCR about 1000bp size that targeted...

02 February 2015 7,712 3 View