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Hello everyone, Do you have experience in the isolation of Th17 cell clone from human PBMC? One common method is to firstly isolate naive CD4+T cells, then to use flow cytometry sorting (CD161...
09 September 2019 4,637 7 View
Hi, I encountered some problems in intracellular staining of IL-17 or IFN-r in my experiments. I cultured PBMC alone or PBMC with PHA at a concentration of 5ug/ml at 6-well plate for 48h, and...
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Hello everyone, In my experiment, I have two conditions: PBMC alone and PBMC co-culture with synoviocytes in 6-well plate for 48h. PBMC were from frozen PBMC, and after thawing , the percentage...
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