June Zhao

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Questions related from June Zhao

How to detect the mRNA expression level from a small number of cells?

I extract RNA from about 30 000 cardiomyocytes with RNeasy Mini Kit(Qiagen), but I can not detect the concentration of RNA. However, GAPDH can be detected with RT-qPCR experiment. At the same...

01 January 1970 2,046 5 View

Dear all.In the animal experiment, how many animals are enough for detection of mRNA expression level?

For example, when our lab do experiment with rats, the numbre of rats of each group is at least 8. And, one rat is enough for detection of mRNA expression level , for example, in heart. My...

01 January 1970 9,531 1 View