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I found too many bands when ~30 microgram protein loaded in the gel from cancer cell lines, but I see very clean band in normal cells! Your precious comments will be appreciated! Thanks
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I am new in culturing the PC-3 cells. I have cultured in RPMI-1640 medium supplemented with 10% FBS. I have plated 200,000 cells per well of 6- well plate.
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