Junaid Ur Rehman

2 Questions 5 Answers 0 Followers

Questions related from Junaid Ur Rehman

Human dermal fibroblasts detach after PFA fixation, cultured in serum-free media?

I lose cells after4% PFA fixation at room temp for 15 mins, cultured in serum free media. I tried PLL coating but it doesn't seem to help much. I tried titrating PFA cocentration and time but...

04 November 2024 1,032 4 View

How to block Fc gamma binding in human macrophages for immunofluorescence?

I tried earlier with 5% Natural Goat Serum but I see a lot of background fluorescence in cells stained with secondary antibody only. I spoke with a few company represenatatives apparently they...

26 July 2023 1,314 9 View