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Hi, i faced some of southern blot problem. my chlamydomonas transformants vector is pChlamy RNA3. and vector has APH8(paromonycin) region. So i designed APH8 probe. probe size is 350bp. After...
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Hello I extracted Chlamydomonas genomic DNA. and i checked gel electrophoresis. But i can't find genomic DNA in gel. and it remained smear. i didn't treated RNase. Can you check my protocol? 1)...
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