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Hi guys. Is there anyone who has the full sequence of plasmids below?? 1. pUTGFP (Tombolini et al., 1997) 2. pDSK-GFPuv (Wang et al., 2006) Thank you! Best, Jun
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Dear All, Is there anyone observed rifampicin color changes when it is treated to bacterial culture? I treated rifampicin to exponentially growing S. typhimurium in LB and after 3 hour, it turned...
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I'm trying to purify my proteins which are cell wall binding domain and I found that it binds to E. coli cell wall. So when I break the cell, my proteins are remained in pellet after...
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Hi, Is there anyone who know about glycosylation of protein could inhibit His6 tagged-protein binding to Ni-NTA resin? I was trying to purify secreted protein from pichia using Ni-NTA resin and I...
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