Jun He

2 Questions 8 Answers 0 Followers

Questions related from Jun He

Does anyone recognize what they are in my cell culture plates? What kind of contamination is it?

I have been doing stable cell lines with knockout genes using CRISPR/Cas9 system for months. I am using ready-for-use plasmids from Santa Cruz. The first step I do co-transfection in target cell...

08 August 2015 1,109 4 View

Help needed for cell culture issue.

Something is wrong in our cell culture system. It has been like this for months. Cells cannot survive after several passages. They are gradually dying. At the beginning, only some stable cells...

05 May 2013 8,008 17 View