Jun-Soon Kim

2 Questions 1 Answers 0 Followers

Questions related from Jun-Soon Kim

Which lysis buffer is suitable for western blot of LAMP-1 ? Can you help me?

Hello, everyone.~ I'm trying to do western blot for LAMP-1 protein. The cell line is 50b11 cell line(rat DRG cells). At first time, I used MPER buffer(thermofischer) and LAMP-1 monoclonal...

25 October 2017 4,548 2 View

In the western blot for LC3B protein in response to the specific chemical, should I use the subcellular fractionation?

I'm trying to do western blot for LC3B protein after the treatment of the specific chemical. In my knowledge, LC3b is a cytosolic soluble protein and become a part of autophagosome membrane in the...

14 May 2017 500 3 View