Julio César Flores González

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Questions related from Julio César Flores González

What is the protocol for staining PBMC with Cholera Toxin Subunit B conjugated with AF488?

Hi! According to the question, in our lab, we are standardizing the CTB conjugated with AF488, but we have not had success. In my experience, I believe it necessary to stain the obtained PBMC...

11 March 2021 2,814 0 View

What is the best time point and stimulation for analysing monocyte expression of IL-10 and TNF-alpha by flow cytometry?

We need to stimulate monocytes by LPS (10 ng) with brefeldin A and monoensin for 4 hrs ( TNF-alpha expression) and 24hrs (IL-10 expression). we are use the whole blood protocol. Thank you for...

17 February 2018 9,681 2 View