Julie A Reynolds

4 Questions 9 Answers 0 Followers

Questions related from Julie A Reynolds

Why does my qPCR look like this?

qPCR assays that previously worked perfectly are not working any more and the results are, in a word, baffling (see picture). The amplification appears to work fine until cycle 30 or 35 and then...

11 November 2019 4,710 1 View

Can anyone help me with miRanda miRNA target prediction results for a non-model insect??

I am trying to identify possible microRNA targets in the genome of a non-model insect using miRanda. I have results, but I don't really know what is considered a good score. I'd love to know what...

02 February 2016 5,145 6 View

Can someone provide any help identifying microRNAs from RNA-seq?

One of the miRNAs identified from my recent miRNA-seq experiment with a flesh fly matches a Bombyx mori miRNA on miRBase. However, a BLAST search with the pre-miRNA sequence suggests this...

12 December 2014 2,465 5 View

I am trying to use western blots to detect methylated histones, but I'm getting really inconsistent results. Is anyone else trying to do this?

I am using a non-Drosophila fly species and I can sometimes get good results with the control, non-modified, histone H3 antibody. But sometimes I don't get any band. I'm not sure if it's a...

10 October 2012 7,659 18 View