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qPCR assays that previously worked perfectly are not working any more and the results are, in a word, baffling (see picture). The amplification appears to work fine until cycle 30 or 35 and then...
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I am trying to identify possible microRNA targets in the genome of a non-model insect using miRanda. I have results, but I don't really know what is considered a good score. I'd love to know what...
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One of the miRNAs identified from my recent miRNA-seq experiment with a flesh fly matches a Bombyx mori miRNA on miRBase. However, a BLAST search with the pre-miRNA sequence suggests this...
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