Julie Hens

3 Questions 8 Answers 0 Followers

Questions related from Julie Hens

Corning MITO+ Serum extender??

Hello, Does anyone have a substitute for Corning® MITO+ Serum Extender ? Or have the specifications for the compounds in it? It seems to be on back ordered forever. Please help. julie

17 April 2023 2,187 4 View

Whats the best way to determine what is binding to the mRNA 3' UTR and blocking a miRNA from binding to it?

I have two cell lines, in one the miRNA binds to its target mRNA, in the other it seems to be block. How do you determine what is blocking the miRNA from binding to the mRNA? Is it protein or RNA?...

22 July 2015 2,899 3 View

How much plasma do I need for Next Generation Sequencing of miRNAs from humans?

I've seen information where 100-200 ul of plasma was used for miRNA RT-PCR, but if I wanted to do next generation sequencing(NGS) on miRNAs from human plasma, how much plasma do I need to start...

23 September 2014 9,256 8 View