What should be the positive and negative controls for gating an assay to detect myeloid and melanoma metastatic cells in the lung?

I am going to perform a flow cytometry assay in order to detect myeloid cells and melanoma metastatic cells in the lung. For myeloid cells I am going to use CD11b antibody conjugated Alexa 488 and...

17 February 2015 2,199 7 View