How can I run in qPCR, reference gene if this gene has a different anneal temperature and PCR conditions than the actual gene I am investigating?

I am new in qPCR, sorry if the question is very basic. I will run B-actin as a reference gene, but the anneal temperature for this gene is different than from Leptin, the gene I am interested in,...

11 November 2015 1,203 3 View