Juliana Félix-Silva

2 Questions 2 Answers 0 Followers

Questions related from Juliana Félix-Silva

In gelatin zimography, which is the best way to use protease inhibitors for identification of specific classes of proteases?

I have a sample rich in proteases. I would like to know if they are metaloproteinases or serineproteases. Many papers employ EDTA or o-phenantroline for metaloproteinases inhibition and PMSF for...

03 October 2016 2,454 2 View

What is the maximum concentration of DMSO that can be used without interfering with the enzymatic activities of snake venom toxins?

I work with snake venom inhibition by plant extracts and I'm having a problem with a specific extract that is very apolar and don't dissolve in water nor PBS nor DMSO 5% in PBS. Is there any...

21 November 2014 9,655 4 View