Hi, I'm unable to sequence a DNA fragment with a polyA region ~13bp. Any advice on if additional Mg2+ may help & suggestions for a gradient protocol?

I am sequencing overlapping regions of the VP2 gene of Parvovirus. We get a good reverse sequence but chromatograms following the polyA region in the forward sequence have very low and mixed...

24 July 2016 9,308 15 View