Julian Trachsel

3 Questions 7 Answers 0 Followers

Questions related from Julian Trachsel

What proteins or genes are commonly used to generate concatenated alignments and phylogenies of bacteria in closely related genera?

I am interested in determining the phylogenetic relatedness of several species of bacteria distributed throughout a few closely related genera. I have already generated a maximum likelihood...

01 January 2017 2,937 2 View

Any advice on two-step PCR library generation with respect to Taq polymerase and 3' A-overhangs?

Hello! I am trying to sequence (Miseq) a custom amplicon from many samples using the Nextera indexes. For those of you not familiar, this involves a two step PCR protocol. The first round of PCR...

01 January 2015 6,289 6 View

Is there a tool available to remove sequences from a protein alignment based on conserved residues?

I am looking for a tool that I can use to remove sequences from my protein alignments that do not contain highly conserved residues. For example, I would be able to direct this tool to remove any...

10 October 2014 4,214 9 View