Can anyone recommend a protocol and antibodies for immunofluorescence for B and T cells in human spleen?

Hi, I am trying to set up a staining for confocal microscopy in human spleen (cryosections). Ideally I would like to stain for CD 3, 4, 8 and CD19 or 20, 27 and 38 (two sets of staining). Can...

13 November 2014 2,607 4 View