Juechun Tang

3 Questions 6 Answers 0 Followers

Questions related from Juechun Tang

Can anyone give suggestion with the binding buffer for immunoprecipitation (or ChIP)??

Hi guys, I need to troubleshoot my IP for low protein in my elutions. I'm performing an IP experiment for a 6X his-tagged protein using an ChIP grade anti-his antibody and dynabeads, I saw...

07 July 2019 9,335 8 View

Did I over-transfer for western blot?

Hi, I used a semi-dry system with 15V 1hr transfer time. The buffer composition was provided by the company who we bought our primary antibody from which is 1.52g Tris, 14.4g Glycine, 100ml...

11 November 2018 1,438 2 View

His-tagged protein CHIP(chromatin immunoprecipitation) Ni-NTA?

Hi all, I have this his-tagged protein which I wanna pull down and want to perform a Chip-seq. While Ni-NTA is used for a lot his-tagged protein purification, I didn't find any citations using...

10 October 2018 8,163 4 View