Juechun Tang

2 Questions 5 Answers 0 Followers

Questions related from Juechun Tang

Can anyone give suggestion with the binding buffer for immunoprecipitation (or ChIP)??

Hi guys, I need to troubleshoot my IP for low protein in my elutions. I'm performing an IP experiment for a 6X his-tagged protein using an ChIP grade anti-his antibody and dynabeads, I saw...

27 July 2019 9,136 7 View

Do people do SDS-PAGE/Western blot for chromatin-immunoprecipitation?

I'm perfomring ChIP-seq now and I noticed that my protein of interest seems to come out from the washes, although some stayed until elution from western blot. However, I don't see people routinely...

12 April 2019 5,663 5 View