Judith Mehrmann

3 Questions 8 Answers 0 Followers

Questions related from Judith Mehrmann

Callose as major component of the cell wall depositions: aniline blue staining?

Hello, everybody, a student of ours wants to show cell wall changes (thickenings) in ultra-thin sections of Arabidopsis (LR White). To detect callose, the sections were stained with 0,01 % aniline...

28 November 2019 2,465 3 View

Does anybody have experience in DAPI staining of Arabidopsis protoplasts?

My goi is located in the nucleus and I cloned it into a YFP vector. The screening at the konfocal was successful and now I want to take a closer look at the nuclei.

06 June 2018 1,655 9 View

Electroporation/transformation in agrobacteria: Problems to successfully get a plasmid into C58C1 and GV3101

Hello all, I have been trying to transform a gene of interest (Arabidopsis gene, in pMDC107 and 163) into Agrobacteria strain C58C1 for quite some time. One construct has a promoter region of...

01 January 1970 8,456 5 View