Juan Rafael Menchú Rosal

7 Questions 5 Answers 0 Followers

Questions related from Juan Rafael Menchú Rosal

What filter paper would you recommend for pre-filtering a solution with lint that's gonna be filtered via membrane for bioburden determination?

I'm determining bioburden of textile materials by filtering on a membrane the diluent used to extract the microorganisms. As it's textiles I'm working with they naturally lose a lot of lint in the...

02 February 2019 5,334 8 View

How is the CFU for Aerobic Count and Yeast and Mold count calculated if extracted from a single dilution?

So I am testing this material for bioburden, I immerse 10 g of the material in 200 mL of eluent and then filter through a 0.45 uM membrane 100 mL for a Total Aerobic Count and 100 mL for a Yeast...

12 December 2018 5,096 7 View

How to improve colorfastness of leather to sweat?

Hi. I've been testing some leather samples for colorfastness to perspiration but they show bad performance. Does anyone know if I should cure them in acid or alcohol or with alkali for it to...

10 October 2018 8,880 1 View

In what step of preparation should pH of a culture media be read?

I've always done it before autoclaving with the media dissolved, but some people recommend doing it with a sample after sterilization with a flat-bottomed pH meter. Anyway I just have a standard...

08 August 2018 1,598 12 View

I am preparing Mineral Salts Agar for textile antifungic activity testing, is it a good idea to have ingredients pre-mixed?

I wanna have the ingredients pre-mixed as to just rehydrate, sterilize and serve it. Do you think it would last or should I prepare it from scratch every time I'm gonna use it? It contains the...

07 July 2018 9,640 4 View

Is anyone familiar with method AATCC 147?

I am trying to validate AATCC 147 but I get different results everytime I run the test, sometimes I get growth under the fabric sample with samples that have shown a halo in previous runs. Any tips?

03 March 2018 4,129 2 View

Does Tween 20 have the same effect as Tween 80 as an antimicrobial neutralizer?

I'm working on antibacterial testing of fabrics and some samples have a water repellent finish. As part of the testing I use Tween 80 to neutralize the antibacterial treatment in fabrics after...

02 February 2018 7,868 6 View