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Questions related from Juan Martinez-Olivan
Mouse IgG1 binding to Protein A Affinity columns is weak. In your experience, what buffer could improve the binding? I read about pH 8.0, 3 M NaCl, some glycerol... Do you have any suggestions?...
11 November 2016 1,901 6 View
We are purifying core Streptavidin (STA). We can obtain the streptavidin from inclusion bodies without major problems but we have difficulties to bind the STA to a Hitrap Q column, even at pH 9.2...
11 November 2016 1,411 3 View
If you want to test the expression of a virus protein in mammalian cells (HEK) and yeasts (P. Pastoris) and you could only optimize the DNA sequence for one of the two cases, which one would you...
11 November 2015 2,114 1 View
We have a protein (34 Kd) fussed in the N-terminal to GST and we are having problems with the binding to a GST affinity column. We tested different pH´s (6-8), buffers (Tris, PBS, phosphate),...
10 October 2015 3,540 4 View
We are expressing a protein by transient tranfection in Hek 293 cells. When performing western blot analysis against our protein of interest (PAH), we obtain two bands, being, probably, our...
03 March 2015 4,520 8 View
We found a band at the expected molecular weight and another 1-2 kD lower with almost the same intensity. We are using an anti-DYK antibody, and our expression plasmid is pcDNA3.1+. The DYk flag...
02 February 2015 7,420 3 View
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02 February 2015 7,867 6 View
