Does anyone know if there is any problem with the expression of Cas9 or in activity if two sgRNA are cloned in a row before Cas9 into the same vector?

Hi all, I'm trying to build a CRISPR/Cas9 single-vector construct but when I perform clones selection I only get clones with two sgRNA in a row. There is a transcription enhancer between these two...

13 June 2016 2,154 1 View