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Currently I am plating human monocytes at a concentration of 1million/mL in a 48 well-plate where I add 400,000 per well. I differentiate them by adding MCSF (100ng/mL) for 6 days (change media on...
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Currently I am working on PBMCs-derived monocyte differentiation into macrophages with mcsf (100ng/mL) for 6 days and I give experimental stimulation for 24 hours. My media is composed of RPMI...
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Currently I am working on PBMCs-derived monocyte differentiation into macrophages with mcsf (100ng/mL) for 6 days and then ifny 25
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I am trying to differentiation human derived monocytes from blood into macrophages. I am culturing them in collagen coated plates (at 0.3 mg/mL) for 7 days. First I culture them for 6 days with...
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