3 Questions 6 Answers 0 Followers
Questions related from Juan José Nieto Fontarigo
We want to do western blot in human serum in order to validate some proteins with changes resulting from iTRAQ-MS/MS data. Is it possible to do western blot directly in human serum without...
23 April 2019 7,800 4 View
We did ultracentrifugation to purify exosomes, but we only obtain 4 ug of protein (BCA assay of intact exosomes) from 2 mL of plasma. This is a very low yield. We need at least 40 ug to do flow...
05 January 2018 6,956 3 View
They were resuspended in 10 mM phosphate buffer but were not resolubilized
25 November 2016 1,717 4 View
