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We want to do western blot in human serum in order to validate some proteins with changes resulting from iTRAQ-MS/MS data. Is it possible to do western blot directly in human serum without...
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We did ultracentrifugation to purify exosomes, but we only obtain 4 ug of protein (BCA assay of intact exosomes) from 2 mL of plasma. This is a very low yield. We need at least 40 ug to do flow...
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They were resuspended in 10 mM phosphate buffer but were not resolubilized
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