Induce Point Mutation in Microbes (RpoB gene for Rifampicin resistance)?

Hi, I try to make Rifampicin resistance microbes (e.g., Corynebacterium) using point mutation in RpoB gene. The doubling time of Corynebacterium is ~3.5hrs, and spontanoeus mutation rate for...

02 April 2018 6,351 2 View