JT Ross

3 Questions 2 Answers 0 Followers

Questions related from JT Ross

What are the best conditions for getting separation between different conformations of HIV-1 RNA, using gel electrophoresis?

Currently I am trying different concentrations of HIV RNA, 1um, 4um, 6um, and 10um. I'm wondering if anyone has tried this perhaps in another RNA virus model and had success; by changing pH or...

29 October 2014 378 4 View

Does utilizing EDTA to stop transcription influence the degradation of RNA?

I currently stop transcription reactions of HIV RNA with EDTA. I am wondering if this could contribute to any unwanted degradative effects as I am seeking the highest yield possible of RNA.

11 September 2014 3,048 3 View

At what percentage is DMSO considered non toxic to cells?

I am working with nuero2a cells and treating them with a protein that has been prepared in DMSO (3)%. I am wondering at what percentage might this DMSO kill my nuero2a cells.

02 May 2013 1,024 9 View