Why am I seeing no insulin-stimulated glucose uptake on 3T3-L1 adipocytes?

I am trying to set up the glucose uptake assay on 3t3-l1 adipocytes. I have tried insulin conc. from 1nM-1uM for 10min, 30min and 2hrs. I just couldn't see any insulin-dependent stimulation. I am...

02 February 2014 2,837 8 View