Why aren't my T cells proliferating with irradiated feeders in culture?

I'm working on a Th17 push assay where I isolate CD4 T cells and culture 1.25 x 10^6 CD4s with αCD3 (2.5 μg/mL), αIL-4 (10 μg/mL), αIFNγ(10 μg/mL), IL-6 20 μg/mL), TGFB1 (5 ng/mL), and IL-23 (10...

02 February 2020 8,141 3 View