Josse Libbrecht

3 Questions 1 Answers 0 Followers

Questions related from Josse Libbrecht

Trouble with lentiviral transduction: GOI not expressed, but cells survive antibiotic selection. What could be the issue?

I have been trying to make a stable cell line expressing my GOI using lentiviral transduction of HEK293T cells. To do so I ordered a lentiviral transfer plasmid from addgene which had a gene in...

27 March 2024 7,368 2 View

Can cellular glutathione interfer with purification of GST tagged protein?

I am trying to purify a GST tagged protein expressed in HEK T cells. Western Blot has shown that the protein is present in the supernatant, but after purification with glutathione agarose beads...

17 January 2023 9,868 1 View

RT-qPCR template concentration: should I dilute RNA of cDNA?

I am currently developing an RT-qPCR assay and I'm about to test my primers. I read that I should use different concentrations of template. I have RNA samples with a concentration of 500ng/µl....

07 April 2022 6,397 3 View