Joshua Hall

3 Questions 3 Answers 0 Followers

Questions related from Joshua Hall

Can I examine chemokines using a western blot from total endothelial cell lysate?

After qPCR analysis of HUVEC lysate from my experiment, I found upregulation of several chemokine mRNA that I would like to validate via western blot. Due to the nature of my experiment, it would...

12 December 2019 8,243 3 View

Why does my HEPES pH changes from 6.9 to 7.5 in 24 hours?

I am adding 25mM HEPES buffer to EGM2 media to culture HUVECs. I initially buffer the solution to 6.9 (I started at 7.1 but I lowered it a bit to compensate for the pH rising), but in just 24...

05 May 2019 3,193 4 View

Difficulty cryosectioning lymph nodes and small intestine?

I am trying to cut 20 um cross sections of murine lymph nodes and small intestines, however, the tissue is fatty and not freezing thoroughly. When I cut the OCT, the cut will have a hole in the...

10 October 2018 2,834 1 View