Joshua Adekunle Babalola

12 Questions 8 Answers 0 Followers

Questions related from Joshua Adekunle Babalola

I did like to know what to do to get a PCR product that will come out with a single clear band, no noise and mixed peak when the product is sequenced?

I am trying to check the polymorphisms of a particular gene from human DNA samples. A set of primer in a published publication allowed the amplification of the DNA hence permitting the analysis of...

03 March 2019 8,376 5 View

What could be the cause of noise or mix in PCR sequence result?

I subjected some genomic DNAs to PCR reaction and later sent then for sequencing to analysis for polymorphisms in my gene of interest? Some of my sequence results came out fine while some came out...

02 February 2019 9,678 7 View

Deglycosylation test: I can't understand why the bands from my deglycosylated proteins are not neat?

I carried out a deglycosylation test to see if there will be difference in the electrophoretic mobility of C57BL/6 infected-ME7 and SShpPrP Tg ( transgenic mice expressing sheep prion protein)...

02 February 2019 5,940 2 View

What could be the likely causes of false positive or false negative results during PCR reaction?

I did tail biopsy to genotype a particular gene. The result came out positive for my desired gene but two days after using same lysed genomic DNA, it came out negative. In my confused state, l...

12 December 2016 5,726 7 View

My transfected cells do grow at sporadic rate a week after subjecting them to antibiotic selection treatment , what could likely cause this?

I am trying to establish a stable cell line expressing a gene of my choice using a prion protein knock out cell line, Lipofectamine and Zeocin as selection antibiotics. Not ruling out the fact...

12 December 2016 9,082 7 View

Please l need recommendation on antibodies that can detect Chronic Wasting Diseases Prion Protein in cell lysate or brain tissue homogenate?

6H4 monoclonal antibody known to be effective in capturing cervids Prion Proteins has been discontinued by Prionics. I need suggestions on antibodies either monoclonal or polyclonal that could be...

12 December 2016 2,387 2 View

Please l need some one to help me with protocol to establish cell line using Lipofectamine LTX plus reagent and G418 as antibiotics for selection?

I need the procedure of how to establish a stable cell line urgently.

08 August 2016 6,639 2 View

Please help with expert views based from experience on establishing cell lines expressing prion protein gene from any of the cervids- elk, deer ?

I need help on the most appropriate transfection approach and conditions to optimize cell transfection when establishing stable cell lines expressing any of the cervids' prion protein genes and...

07 July 2016 2,571 0 View

I did a transient transfection on Zpl neuronal cell line but the transfection wasn't successful. Please l need help on what could have caused this?

I am trying to establish a cell line using Zpl cell line- PrP knock out and LTX/PLUS lipofectamine reagent as transfection reagent.

07 July 2016 369 4 View

For BCA or brain homogenate preparation, what volume of Lysis buffer should be added to weighed cell pellet or tissue when lysing cell or tissue?

Just want to know the volume of lysis buffer to added to weighed cell pellet or tissue during cell lysis.

07 July 2016 4,654 0 View

Can Micro RNA be extracted from tissues?

I need protocols or procedures for extracting Mi RNA from tissues in experimental animals.

05 May 2016 1,209 4 View

Could Murine Endogenous Retroviruses be the cause or consequence of spontaneous neurological symptoms in laboratory models of Prion diseases?

It has been observed that certain laboratory models-- rabbits and few others developed spontaneous neurological symptoms upon inoculation with infectious Prion strains, could Murine Endogenous...

04 April 2016 4,016 2 View