Joshua Julian Glass

4 Questions 6 Answers 0 Followers

Questions related from Joshua Julian Glass

When examining in vitro platelet activation, which is the best anti-coagulant?

Hi All, I am wondering which is the best anti-coagulant to use when activating human platelets by a range of agonists in vitro (by flow cytometry)? I will be incubating the platelets with a range...

15 April 2016 1,743 11 View

Where can I purchase monodisperse fluorescent polystyrene nanoparticles?

Hi All, I would like to purchase a range of fluorescent polystyrene nanoparticles that are quite monodisperse (narrow size distribution) and all within the nano size range. They are for immune...

18 January 2016 5,461 6 View

Washing blood - any quick ways to remove all plasma proteins from blood cells?

Hello All, I have been 'washing' primary human blood to remove plasma proteins from the mixed cell populations in 1x PBS. This involves up to 6PBS washes, where the supernatant is removed after...

15 October 2015 2,528 6 View

Best practice for analysing platelets by flow cytometry?

Hi All! I'm going to be analysing platelets by flow cytometry -- Simple surface staining. Does anyone have advice for separating platelets from blood? The need for platelet buffers? How to spin...

12 February 2015 6,332 11 View