Joshua Aaron

2 Questions 2 Answers 0 Followers

Questions related from Joshua Aaron

Has anyone struggled to select for clone carrying a gene in DH5alpha, noting the vector relies on the T7 system?

Some context: I'm trying to generate a clone possessing a gram positive phage endolysin gene in E. coli DH5alpha. I'm using the pet28 vector and initially tried to clone/transform directly into...

05 February 2024 7,217 3 View

How can one Improve bacteriophage samples for SEM?

Currently, I am struggling to obtain quality SEM images to assist in the classification of phages obtained from sewage samples from hosts E.coli and K.pneumoniae. I have followed the standard...

29 October 2020 7,619 2 View