Joshna Gadhavi

3 Questions 5 Answers 0 Followers

Questions related from Joshna Gadhavi

Western blot protocol

What is the composition of TBS, TBST and transfer buffer to perform semi-dry western blot?

06 June 2017 8,418 4 View

What is the alternative of dialysis step?

I am purifying alpha synuclein protein, in that supernatant is dialysed against tris buffer. to avoid this step what is the alternative of this step?

12 December 2016 3,530 3 View

Why non specific bands are coming after purification of alpha synuclein by osmotic shock method?

I have tried to purify alpha synuclein from DEAE-sepharose column. In that cell pellet are lysed again osmotic buffer. And then pellet collected after centrifugation, was resuspended in cold...

01 January 1970 7,268 4 View