Josephine Swiggs Malmevik

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Questions related from Josephine Swiggs Malmevik

How do I quickly find out whether an AAV plasmid is single-stranded or self-complementary?

Hi all, We have purchased an Addgene plasmid (#61593) and cannot find out in articles referencing it (have not emailed the authors yet) whether this is an ss or sc vector. What is the quickest...

10 October 2016 9,177 2 View

CRISPR efficiency calculations for indel%?

Hi all, We use the Surveyor assay and I have some questions as we get a maximum of 14% indel occurrence. We also have some questions regarding the use of the formula specified by Ran et al 2013 in...

09 September 2016 855 3 View

Intracellular detection of myc using FACS?

Hi all, Does anyone have experience with detecting _intracellular_ myc using FACS (ie not surface-bound)? (We want to quantify the % of cells in culture that contain a myc-fusion protein). Clone...

01 January 2016 9,844 6 View

Is there an optimal way to design a stuffer sequence for insertion into AAV vectors to ensure proper packaging?

To get around 4 kb, would any non-coding sequence be ok? Some use parts of lacz, and some from the pBR322 plasmid etc. Maybe someone has some sequence from the lacz gene (we have this in our lab)...

07 July 2015 8,364 5 View