Josephine Galipon

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Questions related from Josephine Galipon

Nuclear membrane lysis buffer compatible with downstream immunoprecipitation (IP)?

I am performing cytoplasmic/nuclear fractionation of HeLa cells with the objective of performing IP using antibody-conjugated magnetic beads on both fractions. I need to make sure I am not...

11 October 2013 710 1 View

Transfection of human iPS cells: is it possible and what should one be aware of?

I would like to perform over-expression experiments of tagged proteins using pcDNA3.1 as a vector. Keyword searches unvariably give me information on the use of vectors to induce pluripotency, but...

04 October 2013 2,196 9 View