Josephine Galipon

2 Questions 2 Answers 0 Followers

Questions related from Josephine Galipon

Nuclear membrane lysis buffer compatible with downstream immunoprecipitation (IP)?

I am performing cytoplasmic/nuclear fractionation of HeLa cells with the objective of performing IP using antibody-conjugated magnetic beads on both fractions. I need to make sure I am not...

11 October 2013 708 1 View

Transfection of human iPS cells: is it possible and what should one be aware of?

I would like to perform over-expression experiments of tagged proteins using pcDNA3.1 as a vector. Keyword searches unvariably give me information on the use of vectors to induce pluripotency, but...

04 October 2013 2,194 9 View