Joseph Camarano

3 Questions 5 Answers 0 Followers

Questions related from Joseph Camarano

Western Blotting Problem: Detection of actin but not other proteins

I am trying to detect SRC-3. I used primary rabbit antibodies for SRC-3 and beta-actin from Cell Signaling at the suggested dilution of 1:1000 (in freshly made 5% milk). I then used a secondary...

07 July 2017 4,973 4 View

Protocol for immunocytochemistry double staining with only HRP-conjugated secondaries?

I want to perform an ICC double stain for cells cultured on a glass coverslip. I have mouse antibodies for protein A, and rabbit antibodies for protein B, as well as the corresponding antibodies...

07 July 2017 9,724 4 View

MCF-7 aggregating, loosely attached cell clumps. Are they dead cells or contamination?

I have performed a 10 day long treatment with MCF-7 cells in 24-well plates. I have senesced them, and followed by treatments with drugs that are expected to kill normal cells, but also have...

07 July 2017 3,089 5 View