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Questions related from Joseph Camarano
I am trying to detect SRC-3. I used primary rabbit antibodies for SRC-3 and beta-actin from Cell Signaling at the suggested dilution of 1:1000 (in freshly made 5% milk). I then used a secondary...
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I want to perform an ICC double stain for cells cultured on a glass coverslip. I have mouse antibodies for protein A, and rabbit antibodies for protein B, as well as the corresponding antibodies...
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I have performed a 10 day long treatment with MCF-7 cells in 24-well plates. I have senesced them, and followed by treatments with drugs that are expected to kill normal cells, but also have...
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