Joseph Scarrott

2 Questions 1 Answers 0 Followers

Questions related from Joseph Scarrott

Can the citrate in low pH mammalian cell lysis buffers be replaced with a different ion, for instance formate?

I am trying to find more information on low pH acidic lysis buffers to replace a current alkaline lysis process. Most acidic lysis buffers I have seen described contain citrate. I would like to...

11 April 2023 1,944 2 View

Can anyone suggest a cause for a lack of fluorescent signal from a Diorex UltiMate 3000 RS detector in an HPLC experiment?

I am trying to detect viral capsids using an UltiMate HPLC system. I have a strong peak at the expected retention time using a 260/280nm UV detector, however I am not receiving any signal from the...

28 October 2020 3,832 2 View