Joseph Palmer

2 Questions 3 Answers 0 Followers

Questions related from Joseph Palmer

Is restriction digestion necessary before T RFLP?

I've designed a novel Multiplex-T RFLP protocol for the simultaneous detection of a various strains of pathogens from a genus of bacteria. The multiplex design and gene selection mean that each...

04 October 2017 9,436 4 View

Do fluorophores affect the melting temperatures of primers?

I've optimised the PCR protocol for an unlabelled primer pair that target a range of pathogenic bacteria. The primers produce a amplicon of the predicted length at an optimum annealing temperature...

21 April 2016 9,609 2 View