Joseph Lee

3 Questions 2 Answers 0 Followers

Questions related from Joseph Lee

No cytokine or complement activation in my PBMC stimulation assay?

Hi I`m trying to detect immune response in vitro with my nanoparticle (virus-like particles) incorporating SARS-CoV-2 Spike protein. I inoculated quite a lot of nanoparticle in PBMC and inoculated...

10 April 2024 228 2 View

PBMC T cell stimulation by IL-2 vs anti-C28/CD49? Whats the difference.

Hi I`m trying to test the immune response of T cells with some nanoparticle. But I`ve been reading different papers and it seems people either co-stimulate T cells with IL-2 or anti-CD28/CD49....

01 January 1970 3,073 0 View

Should I use anti-CD3/CD28 or anti-CD28/CD49d to stimulate T cells?

I`m trying to observe pro-inflammatory cytokine release in T cells obtained from patient PBMC when exposed to a specific antigen in vitro. But I`m not sure if I should use anti-CD3/CD28 or...

01 January 1970 1,857 2 View