Joseph Philip Bennett

4 Questions 7 Answers 0 Followers

Questions related from Joseph Philip Bennett

What is the best pH to grow Lactobacillus rhamnosus?

Hi all, I'm doing some research in the proteins secreted by L rhamnosus and I've been trying to optimise the fermentation conditions to maximise production of these proteins. Without pH control...

09 October 2020 4,960 6 View

How do can I visualise proteins with high Ala and very low Arg contents by SDS-PAGE?

Hi there, I'm trying to purify some potentially interesting native secreted protein for a bacteria. I can see some good strong peak on the UV trace following IEX chromatrography using an AKTA...

26 February 2020 303 3 View

What equipment do people use for larger scale (+ 1 L cell suspension) cell lysis for recombinant protein recovery?

I am currently working on a project where I need to recover and purify a number of His-tagged proteins for multiple 30 L fermentation runs. At smaller scales (around 1 L ferms) I have used a high...

26 February 2020 4,308 3 View

How can I disrupting protein aggregate before SEC?

I'm trying to isolate and identify a protein from a fungal secretion based on it's activity. I have tried GF using Superdex 75, Superdex 200 and Superose 6 columns but the only peak with activity...

11 July 2016 3,185 8 View